![]() ![]() The result is a visual tree of the matches. The example shown is from BLASTP:Ĭlick on the "Distance tree of results" link. Once again the results are similar for BLASTN and BLASTP. This is activated by clicking the "align two or more sequences" link:Īnother interesting result is the report of the taxonomy tree of the significant matching sequences. The image below is from BLASTP, but the BLASTN has a similar facility. The results show the alignment of the base pairs Compare Multiple SequencesĪnother form of searching is to compare 2 sequences to each other. The results show the amino acid matches Nucleotide (mRNA) These results can be helpful in identifying what the searched sequence matches and what other species have similar substances.Ĭlicking on the name of any of the results will. Ident: the highest percent identity for a set of aligned segments to the same subject sequence.The expect value is the default sorting metric for significant alignments the E value should be very close to zero. E Value: the number of alignments expected by chance with the calculated score or better.There are several types of BLAST searches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families. Query Cover: the percent of the query length that is included in the aligned segments. The program compares nucleotide or protein sequences and calculates the statistical significance of matches.Tot Score: the sum of alignment scores of all segments from the same subject sequence.Max Score: the highest alignment score calculated from the sum of the rewards for matched nucleotides or amino acids and penalities for mismatches and gaps.In either case, the items of interest are: Looking at the section "Sequences producing significant alignments" we see: Analyzing the results of a BLAST search, while similar, will depend on whether the original search was for a nucleotide or amino acid sequence. ![]()
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